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Software for immunohistochemistry quantification


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#1 feifei83

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Posted 23 July 2011 - 11:41 PM

I want to use some software to do semi-quantification analysis of my tissue immunohistochemistry result. I searched on line, and find several software like image pro-plus, tmaj and etc. Could anyone tell me which one is better and accetaple by journals and easy to learn? Thank you very much.

#2 bob1

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Posted 24 July 2011 - 04:02 PM

You can't properly do quantitation on IHC as you have nothing you can use as a standard to measure against, due to differences in distribution of different proteins in the cell. You best bet is to find a pathologist and get them to teach you how to score the slides (or come up with a system of your own). You can make lysates and do it on westerns though.




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