Can i apply primary antibody for two nights instead of one?
Posted 22 July 2011 - 04:00 PM
Posted 22 July 2011 - 08:05 PM
why? cos you got only a weak signal after O/N incubation? or you've to leave the blots over the weekend? or you forgot the blots in the Ab solution over the weekend? there's only one way to find out....so give it a try but you might get higher background.
can i apply primary antibody for two nights instead of one?
- hobglobin, personal comment about my beauteous photo......
Posted 22 July 2011 - 08:26 PM
Posted 23 July 2011 - 11:51 AM