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Can i apply primary antibody for two nights instead of one?


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3 replies to this topic

#1 uvbox

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Posted 22 July 2011 - 04:00 PM

can i apply primary antibody for two nights instead of one?

#2 casandra

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Posted 22 July 2011 - 08:05 PM

can i apply primary antibody for two nights instead of one?

why? cos you got only a weak signal after O/N incubation? or you've to leave the blots over the weekend? or you forgot the blots in the Ab solution over the weekend? ;) there's only one way to find out....so give it a try but you might get higher background.
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#3 Utsav

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Posted 22 July 2011 - 08:26 PM

I think so, it should be fine if you leave your blots with primary antibody in cold room for two nights instead of one. I am telling this because I have done this for my primary antibody and it didn't create any problems while developing the blots.

#4 Curtis

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Posted 23 July 2011 - 11:51 AM

If it's supposed to bind it'll do within 4 hours. 2 nights is not necessary




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