1 reply to this topic

[genie]

Hi All,

Im new to methylation studies and hope to learn more about methylation.
i want to know if the isolation procedure of DNA has an impact on methylation of genes?
should i use column purification kits or can i even used standard phenol-chloroform method for DNA isolation?
Also can anyone tell me if storage of the isolated DNA in -20C can alter the actual CpG pattern of the genes?

thanks in advance.

[MoB]

genie, on 21 July 2011 - 08:14 PM, said:

Hi All,

Im new to methylation studies and hope to learn more about methylation.
i want to know if the isolation procedure of DNA has an impact on methylation of genes?
should i use column purification kits or can i even used standard phenol-chloroform method for DNA isolation?
Also can anyone tell me if storage of the isolated DNA in -20C can alter the actual CpG pattern of the genes?

Nothing will change the methylation status of your DNA, neither any isolation procedure nor your storage conditions.

Best,

MoB