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effect of DNA isolation and storage on methylation


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#1 genie

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Posted 21 July 2011 - 08:14 PM

Hi :) All,

Im new to methylation studies and hope to learn more about methylation.
i want to know if the isolation procedure of DNA has an impact on methylation of genes?
should i use column purification kits or can i even used standard phenol-chloroform method for DNA isolation?
Also can anyone tell me if storage of the isolated DNA in -20C can alter the actual CpG pattern of the genes?

thanks in advance. :rolleyes:

#2 MoB

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Posted 22 July 2011 - 05:28 AM

Hi :) All,

Im new to methylation studies and hope to learn more about methylation.
i want to know if the isolation procedure of DNA has an impact on methylation of genes?
should i use column purification kits or can i even used standard phenol-chloroform method for DNA isolation?
Also can anyone tell me if storage of the isolated DNA in -20C can alter the actual CpG pattern of the genes?


Nothing will change the methylation status of your DNA, neither any isolation procedure nor your storage conditions.

Best,

MoB




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