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Monoclonal Antibody Conjugation


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#1 janeqin

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Posted 20 July 2011 - 03:55 PM

Hi,
I am trying to optimize conjugation with HRP or Biotin for the mouse monoclonal antibody. I use Pierce protocol for biotinylation. For HRP, I used periodate to activate HRP and conjugate with the antibody. But the problem now is the antibody titre is low in Elisa. I am trying to improve its sensitivity. Anybody can give hints to improve the binding capability?

Thanks,
Jane

#2 PAO_ahac

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Posted 25 July 2011 - 03:14 AM

All components of the conjugation procedure have to be optimized.

To increase binding a quick test would be to titer PEG in your immunoassay. It will increase binding...you may also have to increase number of washes and/or amount of surfactant in the washes.




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