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In situ hybridization


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#1 laurentGr

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Posted 24 May 2002 - 05:57 AM

After in vivo transfection, I have to demonstrate that my construction is kept by cells after 1 month. How could I perform this ?
We have already demonstated that cells expressed the protein of interest, but we would like to demonstrate that after a long time, transduced cells conserved the plasmid. What kind of method should we used to perform this?
Thanks

#2 milanoj

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Posted 24 May 2002 - 06:03 AM

If your construct is highly expressed you can try FISH. If you want to answer the question directly in situ PCR is the answer




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