I have run SDS-PAGE gels for some times however i am know facing a big problem
since every time i do a new gel and western blot whith the same samples i am
getting completely different results in the wells, because some bands misteriously
disapear or others stain really black as in the following gel.
I don't know what could be wrong i am doing every thing i used to.
I run the gel at 160V for 1:30min and use running buffer at pH 8.3 with 10% SDS,
but when i finish the western blot i dont get even results and i am getting desperate.
I transfer to a PDVF membrane with CAPS with pH 11.
Could someone please give me some advice, i would really apreciate it.