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Freezing proteins at -80


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#1 bugsbunny66632

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Posted 20 July 2011 - 11:10 AM

Hi,

I used RIPA lysis buffer with protease inhibitors to collect cell lysates, and then I clarified the lysates to get protein concentrations.

Then I put the proteins into the -80 freezer to store. However, I did not snap freeze in the liquid nitrogen tank. I am just looking for total protein levels in the lysate. Do you think not snap freezing will be a problem??

Thanks!

#2 mdfenko

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Posted 20 July 2011 - 12:09 PM

no.
talent does what it can
genius does what it must
i do what i get paid to do




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