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4% paraformaldehyde


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#1 bugsbunny66632

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Posted 20 July 2011 - 11:08 AM

Hi,


When I created fixing buffer for my cells for immunofluorescence, I used 2x PHEM buffer at 1x but diluted it to 1x with PBS instead of water. Since 4% PFA buffer can be made with PBS too, do you think this will be a problem? I'm just worried that the cells might be unhappy with that mix of buffers.

#2 bob1

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Posted 20 July 2011 - 03:14 PM

Adding PBS increased the salt content so the cells might not be happy. You can easily fix in formaldehyde in PBS if you need to make more.




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