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NGF and PC-12

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#1 pana



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Posted 20 July 2011 - 01:56 AM

I'm working with PC-12 floating cells and trying to differentiate them.
I plate them on collagen treated plates and treat them with 50,100,200ngr/ml NGF. According to literature I can see neurits after 3 days, but I'm not seeing any.
Is it something to do with the cells growing in clumps or should I give it more time?

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