Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

SDSPAGE


  • Please log in to reply
No replies to this topic

#1 sahayog

sahayog

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 26 November 2002 - 05:37 AM

Hi
I am encountering a problem in SDSPAGE
Details
when we run the gel without boiling the sample in SDS gel loading buffer we do not get any protein bands in the lane except at the bottom of the gel, where a thick band is observed.whereas if we boil and load the sample as per the std. protocol for 2 min. we get a kind of protein ladder with diminishing of the low mol. wt. chunk.
what could be the reason
help me soon
see you
sahayog




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.