1 reply to this topic

[pm1234]

Hi

I have to treat my cells with EGF for my experiment. The EGF that we have in the lab is already reconstituted to a certain concentration. The tubes don't have any info about when they were made so I have no idea how long they've been sitting around at -20. I have used it for few of my experiments....but I get different results with replicate experiments. I am thinking EGF we have might be suspect. I have no idea where it was purchased from originally and few vendors mention that shelf life after reconstitution might be anywhere from few months upto a year. Was wondering if anyone had any idea about this. Any help is much appreciated Thanks

[protolder]

pm1234, on 19 July 2011 - 06:02 AM, said:

Hi

I have to treat my cells with EGF for my experiment. The EGF that we have in the lab is already reconstituted to a certain concentration. The tubes don't have any info about when they were made so I have no idea how long they've been sitting around at -20. I have used it for few of my experiments....but I get different results with replicate experiments. I am thinking EGF we have might be suspect. I have no idea where it was purchased from originally and few vendors mention that shelf life after reconstitution might be anywhere from few months upto a year. Was wondering if anyone had any idea about this. Any help is much appreciated Thanks

Hola in the web pg of BDbioscience there are recommendatios to store disolved EGF. I hope it serves to you. Buena suerte