Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Is pCL-Eco suitable for packaging viruses that used to infect human cells?


  • Please log in to reply
1 reply to this topic

#1 Yang Gao

Yang Gao

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 19 July 2011 - 03:19 AM

we cotransfected 293T cells with pCL-Eco and pBABE-puro, collected supernatants to infect human 5637 bladder cancer cells.
But failed.
Addgene said about pBABE: "Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC." http://www.addgene.org/1764/
Is it right? why?

Is pCL-Eco suitable for packaging viruses that used to infect human cells?

#2 chabraha

chabraha

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 137 posts
2
Neutral

Posted 22 July 2011 - 10:36 AM

pCL-Eco is only suitable for Rat and Mouse cells..........you need pCL-Ampho or VSV-G for generation of retrovirus capable of infecting human cells
Treasure Chest Wizardry




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.