Posted 18 July 2011 - 02:52 AM
After extract my RNA and ran the gel electrophoresis, I couldnt see any bands for my RNA extraction. However, after synthesized my cDNA (using the same RNA) and did PCR reaction adn ran again the electrophoresis, I could see clear bands with the right size for my housekeeping gene (ubiquitin). I wonder why I cannot see the band at first?
Posted 18 July 2011 - 08:31 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon