Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -


  • Please log in to reply
1 reply to this topic

#1 seed



  • Active Members
  • PipPipPipPipPip
  • 35 posts

Posted 18 July 2011 - 02:52 AM

Can someone explain to me about my result???
After extract my RNA and ran the gel electrophoresis, I couldnt see any bands for my RNA extraction. However, after synthesized my cDNA (using the same RNA) and did PCR reaction adn ran again the electrophoresis, I could see clear bands with the right size for my housekeeping gene (ubiquitin). I wonder why I cannot see the band at first?

#2 Trof


    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,310 posts

Posted 18 July 2011 - 08:31 AM

What conditions did you used for RNA gel? Total RNA is usually run in denaturing gel or buffer, stained with EtBr, then you can see two (three) bands of rRNA (tRNA) and smear around which is mRNA. Was that the first time you run RNA on gel or did it work well before?

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.