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GAPDH


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#1 tulip123

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Posted 18 July 2011 - 01:09 AM

Hi every one. I have used monoclonal antibody to GAPDH and detected a non-specific band at about 100 kd. Has anybody else ever got that? The antibody I am using is one of abcam's. Thanks.
tulip123

#2 bob1

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Posted 18 July 2011 - 03:56 PM

I think you will find that everyone who has done westerns will at some point have had non-specific bands... There are lots and lots of threads on here with information on how to reduce non-specific binding.




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