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1 reply to this topic
Posted 18 July 2011 - 01:09 AM
Hi every one. I have used monoclonal antibody to GAPDH and detected a non-specific band at about 100 kd. Has anybody else ever got that? The antibody I am using is one of abcam's. Thanks.
Posted 18 July 2011 - 03:56 PM
I think you will find that everyone who has done westerns will at some point have had non-specific bands... There are lots and lots of threads on here with information on how to reduce non-specific binding.