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which is the best plasmid vector for cloning BSP products?


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2 replies to this topic

#1 genie

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Posted 18 July 2011 - 12:56 AM

Dear All,
Hi, Im a newbie to bisulphite sequencing. please give me some advice on which is the best plasmid vector for cloning BSP products? and which cells to use for cloning?
are TA cloning vectors like TOPO better than those using restriction enzyme sites.
please suggest a good standard working pair.
Thanks in advance.
genie

#2 pcrman

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Posted 30 July 2011 - 10:07 PM

TA cloning vectors should be used to clone your BSP products and you don't need restriction cloning which will make it harder. Any TA vector should work, I do recommend TOPO.

#3 genie

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Posted 31 July 2011 - 07:22 PM

THANK YOU for the comment :) Epigenetist!




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