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How can I detect mannosidase activity of a protein


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#1 Andy1

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Posted 15 July 2011 - 10:46 AM

As the title says i need help finding a protocol with witch I can detect mannosidase activity of a protein.
Thanks in advance.

#2 klinmed

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Posted 17 July 2011 - 12:57 PM

Hope the attached helps.
If you need to assay a-mannosidase use p-Nitrophenyl a-D-mannoside as substrate at pH 4.5

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