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problem in pcr

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#1 najva



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Posted 15 July 2011 - 09:26 AM

I did my pcr and it had very dood bands but suddenly now It dosent have bands. I dont know what thing changed? Please help . Very thanks.

#2 Adrian K

Adrian K

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Posted 15 July 2011 - 06:46 PM

primers degraded? template degraded? uncalibrated thermalcycler?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

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#3 sciencelover



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Posted 16 July 2011 - 07:23 PM

Are you using good quality [url="[url]http://www.alkaliscientific.com/en/filter-pipette-tips/637-a-barrier-tips.html"]filter[/url] tips[/url] when loading your samples? I found a lot of contamination issues were simply from the plastics I was using. Try the A+ Barrier Tips from Alkali Scientific... they are relativiely inexpensive and they work great.

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