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creat a SY5Y stable cell line


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#1 Andreia Carvalho

Andreia Carvalho

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Posted 15 July 2011 - 03:37 AM

Hi everyone!

I want to creat a stable cell line using SY5Y cells. I will do the transfection with the lentivirus next week and i'll do selection with G418..someone has an idea how long in average does it take until i can freeze them for the first time?

Thanks and good luck with your culturing ;)

Andreia

#2 bob1

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Posted 16 July 2011 - 09:47 PM

Depending on whether you are making polyclonal or monoclonal cell lines, it will take between 3 weeks and 2-3 months.




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