Hi,
Currently I'm doing sticky-end ligation. I cut my vector and PCR product (with BamHI overhang) with BamHI.
So, is it necessary to dephosphorylate my vector to prevent self ligation?
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#2
GNANA
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Posted 15 July 2011 - 01:12 AM
Yes ofcourse, you need to dephosphorylate the vector....
badguy, on 14 July 2011 - 07:53 PM, said:
Hi,
Currently I'm doing sticky-end ligation. I cut my vector and PCR product (with BamHI overhang) with BamHI.
So, is it necessary to dephosphorylate my vector to prevent self ligation?
Currently I'm doing sticky-end ligation. I cut my vector and PCR product (with BamHI overhang) with BamHI.
So, is it necessary to dephosphorylate my vector to prevent self ligation?
I would prefer being perfectionist rather than a passionist in Research.
I always had an alternate hypothesis....
I always had an alternate hypothesis....
#3
badguy
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Posted 17 July 2011 - 05:33 PM
will my insert self ligate after treatment with BamHI?
