Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Protein stability at room temperature


  • Please log in to reply
3 replies to this topic

#1 G1R2

G1R2

    member

  • Active Members
  • Pip
  • 16 posts
0
Neutral

Posted 14 July 2011 - 05:48 AM

Dear Friends

I am a new student in biology field. So i have a stupid question in my mind....can anyone please let me know that if protein samples are kept out of ice while working in the lab by mistake for 2/3 minutes whether there is a chance of protein breakdown or degradation??

How long is it OK if kept out of ice?? :blink:

G1R2

#2 K.B.

K.B.

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 174 posts
3
Neutral

Posted 14 July 2011 - 09:49 AM

The answer is - it depends on the protein.

#3 G1R2

G1R2

    member

  • Active Members
  • Pip
  • 16 posts
0
Neutral

Posted 14 July 2011 - 10:36 AM

The answer is - it depends on the protein.



Thanks a lot KB....I really appreciate your prompt response...

The sample is nuclear extract sample and I am looking for all the glycosylated proteins and Thrombospondin 1..Waiting eagerly for your response....:)

#4 K.B.

K.B.

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 174 posts
3
Neutral

Posted 14 July 2011 - 10:47 AM

I don't know temperature stability of a protein I was working on for last couple of years and you expect me to know yours? :)
Check the literature data.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.