3 replies to this topic

[G1R2]

Dear Friends

I am a new student in biology field. So i have a stupid question in my mind....can anyone please let me know that if protein samples are kept out of ice while working in the lab by mistake for 2/3 minutes whether there is a chance of protein breakdown or degradation??

How long is it OK if  kept out of ice??

G1R2

[K.B.]

The answer is - it depends on the protein.

[G1R2]

K.B., on 14 July 2011 - 09:49 AM, said:

The answer is - it depends on the protein.

Thanks a lot KB....I really appreciate your prompt response...

The sample is nuclear extract sample and I am looking for all the glycosylated proteins and Thrombospondin 1..Waiting eagerly for your response....

[K.B.]

I don't know temperature stability of a protein I was working on for last couple of years and you expect me to know yours?
Check the literature data.