Has anyone ever tried to transfect an antisense oligo into pancreatic cancer cells?
I've tried using lipofectamine as well as lipofectamine with plus reagent but I don't see any decline in my target protein expression.
I've tried transfecting 6 hours and waiting 24 hours post transfection as well as transfecting twice and waiting 24 hours with no luck.
If anyone has any advice...I would be most grateful!
1 reply to this topic
Posted 07 May 2003 - 07:10 AM
Cancer cells are sometimes difficult to transfect. Most of the time, it's linked to the transfection reagent. None are working with any cell ! For cancer cells i'am used to use jetPEI (qbiogene). It's easy to use and performing well. What I love is that this is not liposome based....