
Use of colloidal coomassie stained to western blotting??
#1
Posted 12 July 2011 - 10:45 PM
Can one use a colloidal coomassie stained SDS-PAGE gel for the western blotting?
I mean, once the gel is coomassie stained, then can we destain it with destaining solution and use the same for the western blottin?
Have anyone tried it.
Please give your suggestions about it.
Thank you
#2
Posted 15 July 2011 - 08:38 AM
the coomassie will, at least partially, come off the protein when transferred but will bind to the membrane.
talent does what it can
genius does what it must
i used to do what i got paid to do
#3
Posted 17 July 2011 - 11:40 PM
Hi,the coomassie stain may interfere with the western, especially if you develop with color deposition.
the coomassie will, at least partially, come off the protein when transferred but will bind to the membrane.
What if I completely destain it?
Thank you
#4
Posted 18 July 2011 - 12:55 AM
Cheers!!!
#5
Posted 20 July 2011 - 12:01 PM
can you? i think that may be very difficult.What if I completely destain it?
you can transfer the stained protein (it will destain some during transfer). it's just that it will interfere with seeing color deposited on the membrane. if you are looking for fluorescence or chemiluminescence then you should have no problem.
talent does what it can
genius does what it must
i used to do what i got paid to do
#6
Posted 20 July 2011 - 01:02 PM