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protein pass through column


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#1 soymilk14

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Posted 12 July 2011 - 09:46 PM

Hello guys,

i just want to ask if high molecular weight protein can pass out in a gravity flow column? The pore size of the membrane in the column is 30 uM polyethylene and i wanted to wash my HIs beads coated with recombinant protein in there. I was just wondering whether some of the high molecular weight protein still remain on the column? thanks for your info.

#2 mdfenko

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Posted 15 July 2011 - 06:02 AM

the high molecular weight proteins should pass through the column, with sufficient washing, as long as they haven't precipitated.
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