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Culturing Human PBMC


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#1 WisemanRAX

WisemanRAX

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Posted 12 July 2011 - 08:12 AM

I am trying to find a protocol on culturing human PBMCs but I cannot find one on pub med. Does anyone on here know of a protocol that describes the optimum cell concetration per well in culturing these cells in a 96 well plate for 6 days in order to do a CFSE proliferation assay? Also If you do, do you find that using 10% Human AB serum (with RPMI 1640) or 10% FBS (with RPMI 1640) is better for the culture?




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