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protein precipitation


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#1 yeastresearcher

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Posted 11 July 2011 - 06:51 AM

Hi

I am doing TCA extraction of proteins for isolation of proteins .. i am using glass beads and ribolyzing it and then i am spinning down the sample adn removing the supernatant. i am trying to resusped the pellet in 250 micro ltrs of TCA LB + BME .. It is very difficult to re suspend them in the tca lb. can anybody suggest me regarding this ... when ido western blot with the sample. i am not getting thick signal.. its very weak.. i bioled the sample @ 95 deg for 5 min and spinned them down befre running sds page.

#2 Inmost sun

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Posted 11 July 2011 - 09:15 AM

What I do not understand is why TCA is used for resuspension? For SDS-PAGE a Leammli buffer is to use




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