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help, chromatin immunoprecipitation


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2 replies to this topic

#1 cdna

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Posted 10 November 2002 - 01:45 PM

hi, anybody has done ChIP? i have a few questions that need ur help. where d u get protein-A agarose bead at reasonable price? can we make them ourselves? 2. i have an anti-body for my protein of interest, usually how much anti-body should be used for 10 to the seventh of cells? how to optimize the anibody reaction? i will appreciate if u can give me a complete protocol. thank u

#2 earmstro

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Posted 11 November 2002 - 03:27 PM

Check out this site for a very successful protocol:

http://mcardle.oncol...m/protocol.html

Dr. Farnham and her postdocs/students/techs have had great success (and numerous publications) following the protocols listed.

I do not have any vendor recommendations, though.

-Eric

#3 cdna

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Posted 12 November 2002 - 10:56 AM

thanks for ur reply. it is a wonderful resource and i will check it. regards.




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