competent cells stock
Posted 10 July 2011 - 10:38 PM
Posted 11 July 2011 - 12:34 AM
just grow the bacteria overnight (say some 5ml culture without Ab), next day morning add 500 microlitres of the grown culture into 500 microlitres of Glycerol in LB (70% Gly in LB) mix and freeze. This is how i do.. may be some others would give you even other way to make efficient glycerol stocks...good luck
I always had an alternate hypothesis....
Posted 11 July 2011 - 09:39 AM
For an overnight culture in 500ul LB broth, I add equal volume of "stock A" to make a final volume 1ml with concentration of 20% glycerol stock.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434