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wrong plot amplification in one-step RT- PCR

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#1 sufanuza



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Posted 10 July 2011 - 07:58 PM

Hi everyboody

I am working with double strand RNA isolated from feces using a kit from qiagen. I have checked the concentration and purity by nanodrop (aprox 85 ng/ul).
For detecting virus presence I used different probes and primer concentrations together with quantitec-probe RT PCR (qiagen) and I add 5 ul of RNA sample.
The curve showed in each analysis/test did not have a sigmoideal shape (linear view)but looks like 1/4 of a circle, it seem they never get the plateau stage.

My NTC don't show any curve . what can I do? How can I improve it? I will appreciate any suggestion.
thanks in advance.

#2 Trof


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Posted 13 July 2011 - 06:48 AM

Do you have a screenshot?

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