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Questions about pIRES-hrGFP1a


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#1 vahid_bb

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Posted 10 July 2011 - 02:10 PM

Hi every body,

Plasmid pIRES-hrGFP1a is a product of Stratagen. My link

In this vector there is a MCS for inserting the gene before a FLAg tag.

In the vector manual you will see this sentence: The presence of stop codons in-frame with the 3 FLAG tag must be considered when inserting genes into the MCS. it says you have to put your insert which has the stop codon before 3 FLAG tag. If so, translation will be terminated before Flag tag, how the FLAG tag peptide can be fused by our interested protein as there is a stop codon before the FLAG tag?

I need the squence of this vector but the company provides the map, not the sequence. Does anyone have the sequence information of vector pIRES-hrFGP-1a?

Edited by vahid_bb, 10 July 2011 - 09:57 PM.


#2 zhangj3

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Posted 14 July 2011 - 01:49 PM

Are you sure that's what they mean? It sounds like just to remind you there is a stop codon at SrfI site, this needs to be removed during the cloning.




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