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DH5alpha cells

Started by shrimathi ravikumar, Jul 10 2011 06:21 AM

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4 replies to this topic

#1 shrimathi ravikumar

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Posted 10 July 2011 - 06:21 AM

why are DH5alpha cells always preferred.. what are other common cells available for cloning.

#2 bob1

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Posted 10 July 2011 - 03:50 PM

Hmmm, homework?

#3 shrimathi ravikumar

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Posted 12 July 2011 - 06:59 AM

View Postbob1, on 10 July 2011 - 03:50 PM, said:

Hmmm, homework?
not exactly.. kinda interested in knowing!

#4 bob1

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Posted 12 July 2011 - 03:36 PM

Have a look at the genotype - there are several features that make it suitable.  These are in common with other strains that are frequently used.

#5 qzlabs

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Posted 13 July 2011 - 09:10 PM

View Postbob1, on 12 July 2011 - 03:36 PM, said:

Have a look at the genotype - there are several features that make it suitable.  These are in common with other strains that are frequently used.
recA-: eliminates homologous recombination and endA1-:inactivates an intracellular endonuclease that degrades plasmid DNA in many miniprep methods
For higher yield and more protein, check out the new bioreactors at https://qizhonglabs.com/main/.
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