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[bugsbunny66632]

Hi,

I am making NP40 lysis buffer.  To make 10% or 1% NP40 - do I just dilute the detergent in water?  Also - any thoughts on how to make sodium vanadate?

Thanks!

[bob1]

Usually the detergent is diluted in a buffer of some sort containing salts and something to maintain the pH as part of the lysis buffer.  You could dilute it in water first and then add to a sightly more concentrated buffer so as to bring to the correct concentrations if you wished, but it seems slightly counterproductive.

For orthovanadate preparation, see this paper: Gordon, J.A. (1991) Methods in Enzymology, 201, 477-482.