i have a gst fusion protein with a thrombin cutting site. I just do not understand if it is normal to see the cleaved form of my gst and target protein as early as bacterial lysis? i found the cleaved form of my protein in the supernatant after bacterial lysis. is it possible? or is it because i incubated my bacterial pellet for too long with lysozyme?
Edited by soymilk14, 09 July 2011 - 06:51 AM.