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effect of lysozyme on gst-fusion protein

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#1 soymilk14



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Posted 09 July 2011 - 06:50 AM

hi everybody,

i have a gst fusion protein with a thrombin cutting site. I just do not understand if it is normal to see the cleaved form of my gst and target protein as early as bacterial lysis? i found the cleaved form of my protein in the supernatant after bacterial lysis. is it possible? or is it because i incubated my bacterial pellet for too long with lysozyme?

Edited by soymilk14, 09 July 2011 - 06:51 AM.

#2 xabiarias



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Posted 20 October 2011 - 10:48 PM

couldn't be due to lysozime which its ligand is the peptidoglycane cell wall!!!

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