Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Cloning promoters+luciferase gene in lentiviral backbone?


  • Please log in to reply
No replies to this topic

#1 greenhorn

greenhorn

    member

  • Active Members
  • Pip
  • 20 posts
-1
Neutral

Posted 08 July 2011 - 01:59 PM

Hello everyone,

I need to switch to lentiviruses for my promoter-reporterassays. I have my promoters (not commercially available) in pGL3-vectors at the moment, so we thought to try subcloning the constructs+the luc gene into our lentiviral backbone (pLKO.2 and pWPI.1). But I am not sure if that would be the smartest strategy - has anyone done something similiar or could provide some advice?
I also found lentiviral reportersystems from Clontech and ready-to-buy promoters in lentiviral constructs, but don't know if such a system is of advantage if i need to subclone anyway.

Thanks a lot in advance!!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.