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Non specific product has higher melting Temp than target

Started by likefoods, Jul 08 2011 08:09 AM

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#1 likefoods

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Posted 08 July 2011 - 08:09 AM

I am using EvaGreen (like SYBA Greeen) run qPCR, and got GAPDH  non specific product that has higher melting temp on dissociation curve.
Tm of target product is 81C, the non specific product is 87C
Maybe the primer is too short (68bp), this primer pair passed primer dimer and second structure check.


Could you please tell me your thoughts

Thanks
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