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Dissolving Calcium Chloride in PBS: How to Prevent Precipitation?

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#1 Newaj Abdullah

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Posted 07 July 2011 - 10:08 AM

Hi there,

I am trying to make PBS containing 100 mM CaCl2. However, when I dissolve the correct amount of CaCl2 in the PBS, there is a formation of precipitate. Does anyone know how to prevent this and make 100 mM CaCl2 PBS?

#2 bob1


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Posted 07 July 2011 - 03:18 PM

You are probably forming CaHPO4 which is very insoluble in water... you will have to make a more dilute solution of Ca2+ as you will never be able to overcome this problem.

#3 Inmost sun

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Posted 09 July 2011 - 12:12 PM

in addition to bob1: add stock solution of CaCl2 stepwise to PBS under stiring

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