Hi there,
I am trying to make PBS containing 100 mM CaCl2. However, when I dissolve the correct amount of CaCl2 in the PBS, there is a formation of precipitate. Does anyone know how to prevent this and make 100 mM CaCl2 PBS?
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Dissolving Calcium Chloride in PBS: How to Prevent Precipitation?
Started by Newaj Abdullah, Jul 07 2011 10:08 AM
2 replies to this topic
#2
bob1
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Posted 07 July 2011 - 03:18 PM
You are probably forming CaHPO4 which is very insoluble in water... you will have to make a more dilute solution of Ca2+ as you will never be able to overcome this problem.
#3
Inmost sun
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Posted 09 July 2011 - 12:12 PM
in addition to bob1: add stock solution of CaCl2 stepwise to PBS under stiring
