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RPMI 1640 media


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#1 nano on the brain

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Posted 07 July 2011 - 04:57 AM

Hi...

As per supplier instructions RPMI 1640 media (with L-glutamine and phenol red) needs to be stored in the frige at 4 degree, were as RPMI 1640 media (without L-glutamine and phenol red)must be stored at room temperature. my question is that if i add L-glutamine should the media then be stored in the fidge?

#2 gfischer

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Posted 07 July 2011 - 06:59 AM

View Postnano on the brain, on 07 July 2011 - 04:57 AM, said:

Hi...

As per supplier instructions RPMI 1640 media (with L-glutamine and phenol red) needs to be stored in the frige at 4 degree, were as RPMI 1640 media (without L-glutamine and phenol red)must be stored at room temperature. my question is that if i add L-glutamine should the media then be stored in the fidge?

Yes.  L-glutamine is somewhat unstable at room temperature.
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then heaven will be yours, before you meet your end

#3 nano on the brain

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Posted 12 July 2011 - 03:44 AM

View Postgfischer, on 07 July 2011 - 06:59 AM, said:

View Postnano on the brain, on 07 July 2011 - 04:57 AM, said:

Hi...

As per supplier instructions RPMI 1640 media (with L-glutamine and phenol red) needs to be stored in the frige at 4 degree, were as RPMI 1640 media (without L-glutamine and phenol red)must be stored at room temperature. my question is that if i add L-glutamine should the media then be stored in the fidge?

Yes.  L-glutamine is somewhat unstable at room temperature.

Thanks =)




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