DNA polymerase with single G 3'-end overhang activity
Posted 06 July 2011 - 04:27 PM
I came across a product webpage of GC cloning vector (http://lucigen.com/s...ART-GC-Vectors/). It stated that the non-proofreading DNA pol. (e.g., Taq, Tfl, Tth) that add a single 3´-A to PCR products have the ability to add a single 3´-G to DNA molecules as well. I would like to know under what conditions the enzymes will do so? Will this be the cause of my TA cloning failure??
Thank you very much.
Posted 13 July 2011 - 07:36 AM
I wasn't able to find anything about G overhangs apart from telomerase function.
Maybe the enzyme adds G also in some percentage of products and G-C binding is more stable than T-A, but if you have inefficient TA cloning you may have other problems, as I wrote TA is usually very efficient.
I never trust anything that can't be doubted.
Posted 13 July 2011 - 10:56 AM
Posted 14 July 2011 - 06:40 AM
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