Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

measuring the serum Ig concentration and bacteria infection


  • Please log in to reply
1 reply to this topic

#1 lical208

lical208

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 05 July 2011 - 11:12 PM

Hey guys,

I would like to detect if my KO mice developed immunodeficiency. One assay is comparing the serum Ig concentration of both WT and KO mice. Normally the references suggest using the 7 to 10 weeks mice. Why have to be 7 to 10 weeks old? Are there problems if they are elder or younger? And do I have to use the WT and KO mice with the exactly same age. Would it be a problem if one is one week younger? I used the ELISA to measuring the serum Ig concentration. Would my OD value be too high if > 2.0?

The other thing is, did any of you ever use bacteria, LPS or virus to infect the mice to see the immunodeficiency? What is the difference between these three? And where can I get them?

I am new in this field... Many many thanks for you kind help.

#2 lical208

lical208

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 11 August 2011 - 07:19 PM

The age of mice will matter believe it or not. The age range suggested is appropriate for optimal immune induction. Does this mean younger or older mice will not develop immunity? No. However, age does matter. If you do not intend to look into this experimentally, then I would suggest you stick with the recommendation.

Best,


Protocols and Methods at CellAssays.com


Many many thanks!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.