I plate cells to about 25% and wait until 70~80% confluency before I replenish the media and add DMSO. Problem is, the cells are detaching and/or become terribly sensitive after I replenish the media.
I'm using 1ml of media for each well, so I take 1ml pipette to remove old & add fresh media for each well one by one.
I suspected decreasing temperature might be the culprit, so I've done everything to minimize my time at the bench and to make sure the media is at the right temperature, but the entire cell population in general are in much poorer shape after media replacement. Is there something I'm missing that could be causing this?\
Edited by tk102120, 04 July 2011 - 04:57 PM.