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Organotypic Hippocampal culture


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#1 Raj Banala

Raj Banala

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Posted 03 July 2011 - 10:26 AM

Hi dear Friends,

I am Rajkiran, research scholar from Hyderabad,INDIA. I am working in neuroscience field and I am desperately looking for help from the people who are actively working on Organotypic Hippocampus cell culture and also need guidance as i need to standardize the cell culture protocol for my research, So kindly help me out with my questions.

1. At what age should one prepare cultures in case of Rat model
2. Which is the best suitable media to culture Organotypic hippocampal cells
3. what is the media composition and etc
4. I want to work with B27 supplement, how good does it work?

If you have any protocols or website or articles which I could follow, please let me. Hoping to get your replies. Thanx in advance

Best Regards
rajkiran




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