how to get red of high secondary signal
Posted 02 July 2011 - 09:41 AM
i'm doing western and want to reprobe the membrane with different secondary ab the problem that I still get the previous signal also, I heard that you can block the signal using ponceau instead of stripping,do anyone know protocol?
Posted 02 July 2011 - 11:29 AM
Posted 02 July 2011 - 02:32 PM
Posted 05 July 2011 - 04:02 PM
Are you using HRP or alkaline phosphatase as the secondary conjugate? If HRP, you should be able to use Sodium Azide to permanantly block the activity of the first secondary conjugate.
Basically I was trying avoid stripping...
yes, I do use HRP so do you have the protocol of blocking the first activity?
THANKS a LOT
Posted 05 July 2011 - 06:59 PM