I am using 6% native PAGE to resolve 200-400bp DNA. I am getting a lot of smear especially when the volume of the sample is high. Does anybody make stacking gel for running DNA on PAGE?
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#2
tamarine
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Posted 16 July 2011 - 05:26 AM
coldlab, on 01 July 2011 - 05:59 PM, said:
I am using 6% native PAGE to resolve 200-400bp DNA. I am getting a lot of smear especially when the volume of the sample is high. Does anybody make stacking gel for running DNA on PAGE?
#3
mdfenko
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Posted 18 July 2011 - 11:44 AM
i don't think dna would stack the way that proteins do. stacking is dependent on isoelectric point (pI) and the difference in the pHs of the running and stacking buffers.
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