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Stacking gel for DNA PAGE


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#1 coldlab

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Posted 01 July 2011 - 05:59 PM

I am using 6% native PAGE to resolve 200-400bp DNA. I am getting a lot of smear especially when the volume of the sample is high. Does anybody make stacking gel for running DNA on PAGE?

#2 tamarine

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Posted 16 July 2011 - 05:26 AM

I am using 6% native PAGE to resolve 200-400bp DNA. I am getting a lot of smear especially when the volume of the sample is high. Does anybody make stacking gel for running DNA on PAGE?

you should check my post. some expertise give me the clue. happy hunting.

#3 mdfenko

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Posted 18 July 2011 - 11:44 AM

i don't think dna would stack the way that proteins do. stacking is dependent on isoelectric point (pI) and the difference in the pHs of the running and stacking buffers.
talent does what it can
genius does what it must
i do what i get paid to do




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