# cell count

### #1

Posted 01 July 2011 - 11:42 AM

Also, I am growing cells in 2ml wells. If the same count is for that per ml, does tat mean it is (7X10^6 cells ) X 2 for cells in that well?

### #2

Posted 01 July 2011 - 12:11 PM

I am growing some cells in 96 well plate. My cells are in 200ul total solution in each well. I did a cell count an it 7X10^6 cells. But I guess that is 7X10^6 cells/ml. So, to know how many cells i have in the well, is it 1.5X10^6 cells?

Also, I am growing cells in 2ml wells. If the same count is for that per ml, does tat mean it is (7X10^6 cells ) X 2 for cells in that well?

You said you did a cell count.. but a cell count in what?

We need to know in what volume you did that cellcount...

There is no way to know what the cell count is if you dont know what the volume was that you counted.

### #3

Posted 01 July 2011 - 12:17 PM

### #4

Posted 02 July 2011 - 02:18 AM

### #5

Posted 02 July 2011 - 08:09 AM

Is that not right?

### #6

Posted 02 July 2011 - 02:16 PM

To get total cells multiply by the volume in ml.

1.5x10^6 cells is an awful lot for each well of a 96 well plate - your cells are extremely high density which will be affecting how they behave. Typically you would expect approx 5x10^4 cells maximum out of a well of a 96 well plate, assuming confluent growth.

### #7

Posted 03 July 2011 - 01:09 PM

### #8

Posted 03 July 2011 - 02:37 PM

Thanks for that. Just clarifying, the cells we count by hemocytometer is the cells/ml? So, we can calculate the total number of cells. Suppose in my case, the number of cells counted is in per ml, then I have to calculate no. of cells in 200ul (since they are growing in 96 well plates with 200ul in each well). This is will give me cells per well.

yes.

You just need to "recalculate" it so you get a #cells per 200µl

If you know how many cells there are in 1 ml, its easy to know how many there are in 200µl and if a well is 200µl then yeah, just calculate it for 200µl.

You should look up how a hemocytometer works and how they come to that certain volume... once you undesrtand this (its basic math) you will never need to worry about calculations like this again....

I advice you to check it, see if you understand the concept.

I have the feeling you think that the 1ml is a fixed numbr or something that "magically" comes from somewhere and is a general rule.. its not....

Think about what a cube is, what is the volume of a cube? What is the shape of those cells in a hemocytometer?

### #9

Posted 03 July 2011 - 05:40 PM

Thanks