Hi
I'm going to the basics. Would appreciate if anyone could let me what is the best way to retrieve the 3'UTR sequence of a gene?
Thanks
Submit your paper to J Biol Methods today!
3'UTR sequence of a gene
Started by swatcats, Jul 01 2011 06:51 AM
3 replies to this topic
#2
KevinK
-
- Active Members
-
- 55 posts
Enthusiast
1
Neutral
Neutral
Posted 01 July 2011 - 07:25 AM
Swatcats,
You mightbe able to look at cDNA sequences in a database, but these might not be complete. There may be new ways to do it, but I have used 3' RACE in the past and it does the job well.
Hope this helps.
Kevin
You mightbe able to look at cDNA sequences in a database, but these might not be complete. There may be new ways to do it, but I have used 3' RACE in the past and it does the job well.
Hope this helps.
Kevin
Edited by KevinK, 01 July 2011 - 07:25 AM.
Promega Corporation
Madison, WI
Madison, WI
#4
pcrman
-
- Global Moderators
-
- 1,165 posts
Epigenetist
69
Excellent
Excellent
Posted 08 July 2011 - 08:44 PM
Yes you can use biomart if you want to retrieve one or many genes. If you are interested in just one gene, the easy way is to obtain the refseq for that gene in the NCIB gene database, in the annotation of the genbank file, the ORF is indicated, anything after the ORF is 3'UTR. For example, the ALDOA gene in NCBI has this refseq http://www.ncbi.nlm....ccore/NM_000034 the ORF or CD is between 1008 and 2102, 3UTR is after 2102.
