Intracellular staining for IL-21
Posted 01 July 2011 - 04:56 AM
I just wonder if there is any of you here has done intracellular cytokine staining for IL-21 before? I have tried it for a couple of times of using PMA/ION for stimulation of PBMCs but still could not get any staining. Any advice would be greatly appreciated
Posted 11 July 2011 - 11:19 AM
Also for T cell stimulation it is better to use plate-bound anti-CD3 mAbs together with soluble anti-CD28 mAbs.
Edited by Denis Baev, 11 July 2011 - 11:20 AM.