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Intracellular staining for IL-21

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#1 ceci



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Posted 01 July 2011 - 04:56 AM

Hi there! It is my first post in this forum :)

I just wonder if there is any of you here has done intracellular cytokine staining for IL-21 before? I have tried it for a couple of times of using PMA/ION for stimulation of PBMCs but still could not get any staining. Any advice would be greatly appreciated :)

#2 Denis Baev

Denis Baev


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Posted 11 July 2011 - 11:19 AM

Did you use Golgi-stop reagent (Brefeldin or similar)?

Also for T cell stimulation it is better to use plate-bound anti-CD3 mAbs together with soluble anti-CD28 mAbs.

Edited by Denis Baev, 11 July 2011 - 11:20 AM.

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