Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Intracellular staining for IL-21


  • Please log in to reply
1 reply to this topic

#1 ceci

ceci

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 01 July 2011 - 04:56 AM

Hi there! It is my first post in this forum :)

I just wonder if there is any of you here has done intracellular cytokine staining for IL-21 before? I have tried it for a couple of times of using PMA/ION for stimulation of PBMCs but still could not get any staining. Any advice would be greatly appreciated :)

#2 Denis Baev

Denis Baev

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 53 posts
1
Neutral

Posted 11 July 2011 - 11:19 AM

Did you use Golgi-stop reagent (Brefeldin or similar)?

Also for T cell stimulation it is better to use plate-bound anti-CD3 mAbs together with soluble anti-CD28 mAbs.

Edited by Denis Baev, 11 July 2011 - 11:20 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.