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Plasmid substitution mutation- altered mRNA expression


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#1 Gangwolf

Gangwolf

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Posted 01 July 2011 - 01:57 AM

Hi Everyone!
I am studying a transcription factor with multiple serine phosphorylation sites. The interesting thing is that when I mutate some of the sites from serine to alanine using site directed mutagenesis, some of the mutants when transiently overexpressed in HEK293T cells, show significantly reduced mRNA expression compared to wild type plasmid. What are the possible regulatory mechanisms behind this altered expression?
With my superficial background in transcription regulation, I had no clue that a single or double base pair substitution mutation could have this considerably effects. Id appreciate any comments or suggestions!




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