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ssdna bands on gel appear orange with sybr green II


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#1 chas2000

chas2000

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Posted 30 June 2011 - 01:53 PM

Has anyone experienced ssdna showing up as orange under UV when using sybr green II. I used a mix of sybr I and sybr II in my gel (agarose - urea). The dsdna shows up green when subjected to UV light but the ssdna bands are orange and faint. I've looked around and have found no reason for this. It also occurs when I look at ss plasmids. My gel is agarose (1%, Urea 1M), the buffer is (TAE, Urea 1M) and my concentrations of dsdna and ssdna are fairly high...When I use nondenaturing agarose gels, ss plasmids also show up orange under UV....Any ideas why this is happening would be greatly appreciated....




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