strange and branch like bands on SDS-PAGE
Posted 30 June 2011 - 06:15 AM
Posted 30 June 2011 - 08:48 AM
- hobglobin, personal comment about my beauteous photo......
Posted 01 July 2011 - 10:29 PM
Posted 02 July 2011 - 02:35 PM
Protein samples should never be stored at fridge temperatures unless they are in a lyophilised state. Always store at a minimum of -20 deg C.
Posted 02 July 2011 - 10:32 PM
Posted 03 July 2011 - 07:22 AM
I would suggest:
Change to newly prepared /purchased APS. Use it fresh.
Use a multichannel pipette to load your acrylamide in between the glass when you make your gel. DO not load in one-side.
Use a lower voltage to avoid overheating, better you run your PAGE inside ice-box (I always do that).
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
Posted 03 July 2011 - 10:26 AM
Posted 19 August 2011 - 11:55 AM
Edited by Melvin J Noe Gonzalez, 19 August 2011 - 11:58 AM.