membrane filters for chromatography buffers
Posted 30 June 2011 - 12:47 AM
My question is a rather silly one. we perform ion exchange chromatography and gel filtration chromatography of our recombinant proteins and our buffers are normally acidic pH 4 -5. We use 0.22 µ cellulose nitrate membrane filters [Whatman]to filter our buffers. Our bufffers basically are Na Acetate or Na Phosphate and also we sometimes do filter Tris buffer pH 6 -8
Recently someone suggested mixed cellulose esters for filtration purpose. which of tbe two is a better choice or is there some other membrane filter that can be used for our application kindly guide.
thanks for any suggestions or help.
Posted 15 July 2011 - 05:47 AM
you will want to avoid using membranes that will dissolve in the solvent being filtered or that will leach into your medium.
you probably won't want to filter a protein solution through a cellulose nitrate filter.
for simple buffers you can use whichever you find fit.
here is a page that will guide you: millipore membrane resource center
and here is a compatibility guide (sorry, it doesn't include cellulose nitrate): filtration- chemical compatibility.pdf 803.54KB 3837 downloads
Edited by mdfenko, 15 July 2011 - 05:53 AM.
genius does what it must
i do what i get paid to do